D cells had been contributing towards the enhanced numbers (Figure 3–figure supplement 1B and C). We thusCampbell et al. eLife 2018;7:e30947. DOI: https://doi.org/10.7554/eLife.four ofResearch articleImmunologyAC57BL/6 Na e9.90.BALB/c Na e29.70.F4/80hi – Naive F4/80hi – Infected F4/80lo – Naive F4/80lo – Infected Monocytes – Naive Monocytes – InfectedInfected MHC II MHC II9.90.Infected64.35.Naive C57BL/6 Infected C57BL/6 Naive BALB/c Infected BALB/cF4/80 B – C57BL/F4/80 C – C57BL/100********100***Freq. of MGATA6+CD102+11 8 5060 40 2060 40 2060 40 20Days pi283550Days pi Age (Wks)1150Age (Wks)D – BALB/c100E – BALB/c**********CD102+100 80 60 40 20***Freq. of MGATA6+60 40 2060 40 2011 Days pi Age (Wks)283550Days pi501150Age (Wks)Figure two. Residency is maintained in resistant C57BL/6 mice and lost in susceptible BALB/c mice. (A) Representative FACS plots of MF subpopulations from the pleural cavity of naive and d35 pi C57BL/6 or BALB/c mice. Blue: F4/80hi, Green: F4/80lo, Red: monocytes. Percentage of F4/80hi, F4/80lo and monocytes contributing for the MF compartment as a complete in (B) C57BL/6 and (D) BALB/c mice. MF expression of GATA6 and CD102 in naive and L. sigmodontis infected (C) C57BL/6 and (E) BALB/c mice. (A, B, D) Presented would be the information from two separate time course experiments (day 11 and 28 and day 35 and 50), every of which is representative of 3 independent experiments with 6 mice/group/time point.4-Bromoisoquinolin-5-ol Chemical name (C and E) Presented are the pooled data from three independent experiments.Price of 1354952-28-5 **p0.01, ***p0.0001, ****p0.00001 as determined by a 2-way ANOVA on every time point. Error bars represent the imply SEM. DOI: https://doi.org/10.7554/eLife.30947.004 The following figure supplements are out there for figure 2: Figure supplement 1. GATA6 and CD102 expression on pleural myeloid cells. DOI: https://doi.org/10.7554/eLife.30947.005 Figure supplement two. Phenotyping of pleural cavity myeloid cells illustrates differences amongst BALB/c and C57BL/6 macrophage populations through L. sigmodontis infection. DOI: https://doi.org/10.7554/eLife.30947.Campbell et al. eLife 2018;7:e30947. DOI: https://doi.org/10.7554/eLife.five ofResearch articleImmunologyABF4/80hi BMDCKi67hi+ (Freq of F4/80hi)****100 80 60 40 2040 30 20 10F4/80hi (1X106)Day piDay piIsotypeHostDonorDDay 35 / 23 weeks Na e InfectedNa eInfected1010101010101010GATA6 E Day 50 / 25 weeks Na e Infected Na e InfectedCD10101010101010101010101010101010GATA6 FTIM4+ (Freq of F4/80hi)100 80 60 40 20CD102 Day 35 / 23 weeks Na e InfectedG**** ****1010101010101010TIM4 Day 50 / 25 weeks Na e InfectedNaive Infected10101010TIMFigure three.PMID:26644518 Regional proliferation accounts for enhanced F4/80hi cell quantity in resistant C57BL/6 mice. (A) Expression of higher levels of Ki67 by pleural F4/80hi MF from naive (grey bars) or infected (black bars) C57BL/6 mice at d11, d28, d35 and d50 pi. (B) Percentage of Bone Marrow Derived (BMD) cells contributing to F4/80hi population at d35 and d50 pi in naive and L. sigmodontis infected partial bone marrow chimeric C57BL/6 mice. (C) F4/80hi cell Figure 3 continued on next pageCampbell et al. eLife 2018;7:e30947. DOI: https://doi.org/10.7554/eLife.six ofResearch post Figure 3 continuedImmunologynumber at d35 and d50 pi from animals in (B). (D and E) Expression of GATA6 and CD102 by host/donor derived F4/80hi MF at d35 and d50 pi (F) expression of TIM4 by F4/80hi MF at d35 and d50 pi in naive and L. sigmodontis infected partial bone marrow chimeric C57BL/6 mice (G) Expression of TIM4 at d35 and d50 pi.